• BIO4Africa
    Research project at Celignis





BIO4Africa

"Diversifying Revenue in Rural Africa through Circular, Sustainable and Replicable Bio-based Solutions and Business Models"


ProgrammeHorizon Europe, CE-SFS-36-2020
CategoryResearch and Innovation Action (RIA)
StatusCompleted
Period2021 - 2025
Partners25
Budget€9.00m
LinksWebsite, Cordis, Twitter, LinkedIn, YouTube, Facebook
BIO4Africa is a four-year €9m RIA (Research and Innovation Action) project, funded by the EU's Horizon Europe programme involving 25 partners from the EU and Africa. It focuses on the demonstration of sustainable, circular solutions and business models, suitable for African countries, based on the valorisation of a variety of local feedstocks.
BIO4Africa community picture

Map showing countries involved in the bio4africa project

Celignis is an important partner in the project, having a key role early-on with regards to the compositional analysis and evaluation of a wide variety of different local feedstocks. These data will allow decisions to be made with regards to which feedstocks are suitable for which technologies leading to a subset of feedstocks being selected for processing.

The technologies that will be deployed in the project include: pyrolysis (for the production of biochar); hydrothermal carbonisation; and a green-biorefinery (the GRASSA process, which has previously been demonstrated in Ireland in the Biorefinery Glas project).

After the most appropriate feedstocks have been selected for each technology, samples will be sent to the European technology providers where initial tests will be undertaken in order to understand how these feedstocks behave, allowing for fine-tuning and process modifications to improve process efficiencies for each feedstock.

Following these trials arrangements will be made for the equipment to be shipped to Africa where the technologies will be deployed at a number of locations, processing locally-available biomass.

Celignis will also play an important role in the project at these stages, being responsible for the analysis of the outputs (e.g. biochar, HTC char, press-cake, etc.) of the various technologies. The technologies that will be deployed in the project include: pyrolysis (for the production of biochar); hydrothermal carbonisation; and a green-biorefinery (the GRASSA process, which has previously been demonstrated in Ireland in the Biorefinery Glas project).

BIO4Africa project partners

Publications from BIO4Africa Including Celignis Data

Abdeldayem, O.M., Dupont, C., Ferrasa, D. and Kennedyab, M. (2025) An experimental and numerical investigation of secondary char formation in hydrothermal carbonization: revealing morphological changes via hydrodynamics, RSC Advances 15: 12723-12738

Link

Hydrothermal carbonization (HTC) research has mainly focused on primary char production, with limited attention to secondary char, which is formed through polymerization and condensation of dissolved organic compounds in the liquid phase. This research aims to address this gap via an experimental investigation of the impact of stirring on the mass and carbon balance of HTC reaction products, surface functional groups, and surface morphology of secondary char, using fructose as a model compound. A 3D hydrodynamic simulation model was developed for a two-liter HTC stirred reactor. The experimental results indicated that stirring did not significantly influence the pH, mass, carbon balance, and surface functional groups of secondary char produced under the range of experimental conditions (180 C, 10% biomass to water (B/W) ratio, and a residence time of 0-120 min) studied. Nonetheless, it was observed that a stirring rate of 200 rpm influenced the morphology and shape of the secondary char microspheres, leading to a significant increase in their size i.e., from 1-2 um in unstirred conditions compared with 70 um at a stirring rate of 200 rpm. This increase in size was attributed to the aggregation of microspheres into irregular aggregates at stirring rates > 65 rpm and residence times > 1 h. The hydrodynamic model revealed that high turbulence of Re > 104 and velocities > 0.17 m s-1 correlated with regions of secondary char formation, emphasizing their role in particle aggregation. Particle aggregation is significant above a stirring rate of 65 rpm, which corresponds to the onset of turbulent flow in the reactor. Finally, a mechanism is proposed, based on reactor hydrodynamics under stirred conditions, that explains secondary char deposition on the reactor walls and stirrer.

Abdeldayem, O.M., Dupont, C., Ferras, D., Ndiaye, L.G. and Kennedy, M. (2024) Reconsidering lab procedures for hydrothermal carbonization of biomass: The impact of pre-drying and stirring, Journal of Analytical and Applied Pyrolysis 179: 106459

Link

Although industrial hydrothermal carbonization (HTC) uses wet feedstock, lab-scale studies tend to dry the feedstock under the assumption that the rehydration of the feedstock would restore its original properties. To the best of our knowledge, this assumption has not been thoroughly examined at the lab scale; therefore, its investigation is crucial to prevent any discrepancies that might affect the upscaling of HTC. This research aims to examine the effects of pre-drying biomass by comparing it to the use of wet biomass in HTC experiments, employing three different types of biomass (rejected tomatoes, rejected apples, and digestate). Additionally, the study investigates the influence of stirring on pre-dried and wet biomass under the selected HTC conditions. The results indicate a substantial disparity in studied hydrochar properties when using pre-dried biomass compared to wet biomass. For pre-dried biomass, there is a tendency for an increase in mass yield and solid carbon yield in most examined samples (5-10% dry basis) compared to the wet biomass. Regarding functional groups, wet tomatoes and apples exhibit more pronounced peaks than pre-dried samples. Conversely, digestate shows similar spectra across all examined scenarios. The effect of stirring appears insignificant for most of the studied scenarios; nevertheless, it reduced dehydration and decarboxylation reactions during HTC.

Other Celignis Research Projects Funded by the Horizon Programme

Current Projects


Enxylascope aims at bioprospecting and producing a novel set of xylan debranching enzymes, thereby demonstrating its ability to make xylan a key ingredient in a variety of consumer products. Celignis is playing a key role, being the technical lead and responsible for the extraction and modification of xylan from biomass. Further info...

BIONEER, is an Innovation Action project funded by the CBE-JU, under topic HORIZON-JU-CBE-2023-IA-06 (Selective, Sustainable Production Routes Towards Bio-Based Alternatives To Fossil-Based Chemical Building Blocks). This project will start in May 2024 with Celignis, an SME partner and full industry BIC member, playing a leading role in the scaled-up (1 m3) production of platform chemicals.

Further info...

PROMOFER, is an Innovation Action project funded by the CBE-JU, under topic HORIZON-JU-CBE-2023-IA-03 (Improve Fermentation Processes (Including Downstream Purification) To Final Bio-Based Products). This project started in June 2024 with Celignis, an SME partner and full industry BIC member, playing a pivotal role in the project. Our core activities include undertaking the pre-treatment and hydrolysis of lignocellulosic biomass at scaled-up (TRL7, 1 m3) volumes. The resulting sugars are then provided to other partners for downstream fermentations.

Further info...

MANUREFINERY, is an Innovation Action project funded by the CBE-JU, under topic HORIZON-JU-CBE-2023-IA-01 (Small Scale Biorefining In Rural Areas). This project will start in September 2024 with Celignis, an SME partner and full industry BIC member, involved in the analysis of feedstocks and products of the process. We are also contributing towards the technoeconomic analysis (TEA) of the technologies.

Further info...

Concens the development of an integrated biorefinery to make a portfolio of bio-based solvents from 2G sugars and furans obtained from spent coffee grounds and woody biomass. Celignis's activities include (i) Design and optimisation of a high-cell-density, two-stage Clostridial process for butanol from hydrolysates; (ii) in-situ solvent recovery via membrane/ extractive set-ups; (iii) enzymatic hydrolysis of both feedstocks.

Further info...

WoodVALOR valorises contaminated/post-consumer wood waste (WW) via: (i) thermal conversion to biochar; and (ii) fractionation followed by conversions to paints & coatings ingredients. Celignis is involved in Chemo-enzymatic fractionation of decontaminated wood (DW) to sequentially extract/purify lignin and hemicellulose, and in developing hemicellulose-based emulsifiers/stabilizers and binder monomers for industrial formulations. Additionally, Celignis is involved in metals/mineral recovery from decontamination wastewater using biochar produced in the project.

Further info...

LIGNOFUN funnels lignin streams into high-value aromatics. Celignis leads WP4, putting crude low molecular weight (LMW) lignin through integrated membrane and solvent fractionation into fractions for bioactivity screening (antioxidant, UV-absorbance, antimicrobial, emulsion stabilization), followed by enzymatic/chemical grafting (e.g., sugars, fatty acids) to tune solubility/surface functionality and stability.

Further info...

Develop and demonstrate bio-based construction products (e.g., wall panels, flooring tiles, sandwich structures). Celignis leads materials development: chemo-enzymatic extraction and fractionation of lignin/xylan/cellulose from agri/forest residues, followed by advanced chemical modification of xylan and lignin (mixed-molecule grafting, acylation and silylation) to tailor hydrophobicity, flow/rheology (via DP control), mechanical strength, and fire resistance for injection-moulding/3D-printing.

Further info...

Completed Projects


PERFECOAT, a RIA project funded by the BBI JU, targets the development of novel sustainable coatings that will ultimately be available to the public. Celignis is responsible for the extraction and modification of polymers (xylan and chitosan) that will be used as binders in these coatings. Further info...DIBANET was an FP7 research project, written and coordinated by Celignis founder Dan Hayes, that concerned the production of second generation biofuels from biomass feedstocks in Europe and Latin America. The advances in the state of the art of biomass analysis made in DIBANET led to the formation of Celignis. Further info...The BIOrescue project was focused on developing technologies to convert spent mushroom compost, a problematic waste of the mushroom industry, to high value products. Within the project Celignis undertook a compositional profiling study of the feedstocks and developed rapid analysis models for these and process outputs. Further info...The UNRAVEL project (UNique Refinery Approach to Valorise European Lignocellulosics) is focused on the optimsiation of a biomass pre-treatment technology. Celignis plays a key role by analysing and evaluating the extractives present in a wide variety of feedstocks and determining how they influence pre-treatment. Further info...The focus of ENABLING was on supporting the spreading of best practices and innovation in the provision of biomass for the Bio-Based Industry (BBI). Celignis played a key role in the project with regards to stressing the importance of biomass composition in terms of evaluating feedstock and technology suitability. Further info...Celignis was the sole partner in SAPHIRE. The project, funded by the European Union's INNOSUP programme, concerned the production of high-value hydrogels from lignocellulosic residues. These are expected to have applications in the cosmetic and pharmaceutical sectors. Further info...VAMOS concerns the construction and operation of a demonstration-scale biorefinery producing lactic acid from the paper fraction of municipal waste. In the project Celignis will develop custom NIR models for the rapid at-line on-site analysis of the feedstock and process outputs. Further info...This demo project involves innovative superheated steam processing of unwanted bush and invasive biomass into high-value, clean-burning, low-cost solid biofuel. Celignis will analyse feedstocks, and process outputs, and use our QTOF-LC/MS system to profile the steam condensate for high value chemicals. We will then develop a method to recover target constituents. Further info...This was a Marie-Curie Individual Fellowship (MCSA-IF) involving Celignis's Lalitha in which algae were used to recover nutrients from anaerobic digestion (AD) process streams. Further info...

Other Celignis Research Projects

Completed Projects


Celignis is the sole partner in STEAME, a project funded by the Irish Research Council and focused on the development of technologies to make anaerobic digestion more financially viable in Ireland.

Further info...

Celignis News Articles on BIO4Africa

June 1st 2021

BIO4Africa Project Officially Starts


The project focuses on the deployment of several biomass processing technologies in a number of African countries

Today marks the first official day of the BIO4Africa project. It is a four-year €9m RIA (Research and Innovation Action) project, funded by the EU's Horizon Europe programme involving 25 partners from the EU and Africa. It focuses on the demonstration of sustainable, circular solutions and business models, suitable for African countries, based on the valorisation of a variety of local feedstocks.

Celignis is an important partner in the project, having a key role early-on with regards to the compositional analysis and evaluation of a wide variety of different local feedstocks. These data will allow decisions to be made with regards to which feedstocks are suitable for which technologies leading to a subset of feedstocks being selected for processing. The technologies that will be deployed in the project include: pyrolysis (for the production of biochar); hydrothermal carbonisation; and a green-biorefinery (the GRASSA process, which has previously been demonstrated in Ireland in the Biorefinery Glas project).

After the most appropriate feedstocks have been selected for each technology, samples will be sent to the European technology providers where initial tests will be undertaken in order to understand how these feedstocks behave, allowing for fine-tuning and process modifications to improve process efficiencies for each feedstock. Following these trials arrangements will be made for the equipment to be shipped to Africa where the technologies will be deployed at a number of locations, processing locally-available biomass. Celignis will also play an important role in the project at these stages, being responsible for the analysis of the outputs (e.g. biochar, HTC char, press-cake, etc.) of the various technologies.

Click here to read more about the project and Celignis's involvement in it.





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