Lactic acid bacteria (LAB) are industrially-recognised microorganisms for their lactic acid applications and for their probiotic benefits. Although the process is a well-known fermentation
that has been followed for a number of years, lactic acid bacteria suffer from substrate-inhibition and product-inhibition. Product inhibition is considered to be a key reason for not achieving biomass yield
and maximum theoretical conversion efficiency.
Conventionally, lime is used to reduce the product inhibition, but this process results in very high amounts of solid and liquid wastes that are high in salts and need to be treated before disposal.
Extractive lactic acid fermentation by using liquid-liquid extraction systems, membranes, adsorption resins in fed-batch fermentation mode is gaining attention as it takes care of the product and substrate inhibition.
Lactic acid bacteria are hetero- and homo-fermentative. However, this is not straightforward as, under slow growth rates, mixed acids will be formed even with homofermentative bacteria.
It is important to select the strains that are homo-fermentative, have no racemase activity, and are tolerant to inhibitors that are present in industrial by-product streams that have
significant amounts of: starch e.g. starch production and processing plants (potato, cassava, sweet potatoes etc); sugars (e.g. lactose whey permeate); and cellulose (e.g. paper industry streams).
How Celignis Can Help
At Celignis we have expertise and experience in screening lactic acid bacteria for the selection of substrate- and product-tolerant strains.. We can also develop:
fed-batch strategies to achieve high cell mass, and in situ product recovery techniques to separate lactic acid from the fermentation broth. Our experts
will work with you and develop bespoke lactic acid fermentation methods for your feedstock or industrial waste streams.
Propionic acid can be produced from a variety of substrates such as glucose, ethanol, lactose, glycerol, and pectin. So, several industrial streams
will be suitable to produce propionic acid, if the bacteria are adapted to the inhibitors present in the waste streams and fermentation is optimised to
achieve high cell densities and high product concentration.
We can perform anaerobic fermentations and develop fermentation strategies to achieve high cell mass and in-situ product recovery techniques.
We can screen your feedstock for propionic acid production, adapt the strain to any inhibitors present in the feed, and develop bespoke fermentation
and product recovery processes.
Butyric acid is biologically produced by Clostridium species and like other acids (acetic acid, lactic acid, propionic acid),
it is toxic to the bacteria after a certain concentration. Hence, the product titres are generally low which makes downstream expensive.
In order to reduce these costs, in situ removal of butyric acid can be developed. In situ removal strategies are not yet industrially applied for butyric acid, but
it is a key area where progress has to be made to make the process economically sustainable.
At Celignis, we have strong expertise in Clostridial fermentation. We can isolate and or adapt the strains that are suitable for your feedstock and can
develop fermentation strategies to reduce substrate and product inhibition. We will innovate with you for you.
Butanol fermentation is also one of the difficult fermentation pathways due to substrate and product inhibition. However, this can be
avoided by fed-batch fermentation and in-situ stripping of butanol. Also, reducing the feedstock and enzyme costs will make the process more
economically viable. Through using industrial waste streams (negative costs), enzymatic cocktails tailored for the feedstock (allowing low-enzyme dosages), and
with high sugar yields, the right choice of microbial strain, and an effective in-situ removal technology, it is possible to develop an economically-viable butanol process.
At Celignis, we have considerable expertise in Clostridial fermentation and especially butanol fermentation.
Our Chief Innovation Officer Dr Lalitha Gottumukkala has extensively worked in this area and has isolated novel strains and developed novel
methods for non-acetogenic butanol fermentation as part of her PhD.
Natural microbes that produce 1,3-Propanediol are Klebsiella, Clostridia, Citrobacter, Enterobacter
and Lactobacilli. They all use glycerol as a carbon source and produce 1,3-PDO through 3-hydroxypropionaldehyde route
using glycerol dehydratase enzymes and 1,3-propanediol oxidoreductase enzymes.
At Celignis, we have expertise and experience in performing anaerobic fermentations
and developing fermentation strategies to achieve high cell mass and in situ product recovery techniques. We can screen your feedstock for 1,3-Propanediol production,
adapt the strain to any inhibitors present in the feed, and develop bespoke fermentation and product recovery process.
PHA is one of the most complicated fermentation processes, but the possibility to use mixed microbial cultures and the avoidance of sterilisation costs
makes it an interesting process to produce bioplastics. Also, PHA blends are becoming more and more popular to increase the tensile strength and
flexibility of the polymer, possible by using mixed culture substrates.
At Celignis, we have experience in enrichment of desired microorganisms, fed-batch and continuous fermentations
with cell-recycling. We can design and develop the most suitable process for your feedstock by using mixed or mono-culture fermentations.
We can also develop cost-efficient downstream processing steps for efficient PHA extraction by using non-toxic and environmentally friendly techniques.
Yeast fermentation is one of the oldest fermentations and is used in everyday life to produce a variety of commodity products including bread, beer, wine,
cheese, and soy sauce. A few decades ago, yeast gained popularity as an industrial strain for biorefinery and biofuel applications.
Algal cultivation is complicated and requires optimisation to achieve high biomass yields. Algal biomass production depends on nutrient uptake and
other environmental conditions such as temperature, pH, salt concentration etc. It is important to select the strain based on the type of
production (open ponds, photobioreactors), feedstock and application. We have particular expertise in the evaluation and optimisation of algae thorugh
our Chief Innovation Officer, Lalitha, who is currently undertaking a Marie-Curie funded project at Celignis on this topic.
We are available to answer any questions you may have on how to get high value chemicals and biofuels from biomass through fermentation processes.
Just get in touch with us by sending us an email firstname.lastname@example.org, giving us a call at (+353) 61 371 725, or through
our contact form.
Read about the wide variety of analysis packages we have for biochar
Click here to read about the different analysis packages that Celignis offers for the evaluation on biochars. These analyses cover properties relevant to a wide variety of applications, including soil amendment, carbon sequestration, bioenergy, and biomaterials.
For a short period we are offering two TGA analyses for the price of one!
To celebrate the arrival of our thermogravimetric (TGA) equipment, we are offering, for a limited time period, two TGA analyses for the price of one. Click here to read more about TGA analyses at Celignis and to see the various packages on offer.
To avail of this special offer please mention the code (TGA-AUGUST) in an email or when placing an order via the Celignis Database.
Celignis is a partner in 3 ongoing CBE projects: UNRAVEL
and PERFECOAT are RIA (Research and Innovation Action) projects, whilst VAMOS is an Innovation Action project.
Additionally, Celignis was a partner in the BIOrescue RIA project which was completed in 2019.
Thanks for contacting us. One of our representatives will be in contact with you shortly regarding your inquiry. If you ever have any questions that require immediate assistance, please call us at +353 61 371 725.
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