Analysis of Alanine
Alanine (2-aminopropanoic acid) is non-polar, neutral, alpha-amino acid with aliphatic residue.
Alanine is present in L and D configurations. L-alanine is widely distributed as a constituent of protein in all organisms,
while D alanine has been found in some tissues of crustaceans. Additionally, beta-alanine (3-aminopropanoic acid) is present in
the skeletal muscle of vertebrates.
Fermentative production of alanine was reported from molasses with Microbacterim ammoniaphilum mutant strain resistant to arginine hydroxamate. Beta-Alanine can be produced from L-aspartate by cloning and expression of bacterial L- aspartate-?-decarboxylase enzyme in E. coli.
Red algal species belonging to the genus Porphyra and Gracilaria are particularly rich source of free alanine. Alanine content varies with the phases of the life cycle of red algae. Alanine concentration was observed highest in blade form of Porphyra species rather than in conchocelis. The distinct umami flavour of seaweed is caused by the presence of free amino acids including alanine.
Beta-alanine is a common ingredient in sports nutritional formulations because the beta-alanine supplementation leads to improved muscle buffering and reduced muscular fatigue by increasing the biosynthesis of muscle carnosine peptide.
Fermentative production of alanine was reported from molasses with Microbacterim ammoniaphilum mutant strain resistant to arginine hydroxamate. Beta-Alanine can be produced from L-aspartate by cloning and expression of bacterial L- aspartate-?-decarboxylase enzyme in E. coli.
Red algal species belonging to the genus Porphyra and Gracilaria are particularly rich source of free alanine. Alanine content varies with the phases of the life cycle of red algae. Alanine concentration was observed highest in blade form of Porphyra species rather than in conchocelis. The distinct umami flavour of seaweed is caused by the presence of free amino acids including alanine.
Beta-alanine is a common ingredient in sports nutritional formulations because the beta-alanine supplementation leads to improved muscle buffering and reduced muscular fatigue by increasing the biosynthesis of muscle carnosine peptide.
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Analysis Packages for Alanine
Equipment Used for Alanine Analysis
Ion Chromatography
A Dionex ICS-3000 system that is equipmed with electrochemical, conductivity, and ultraviolet-visible detectors.