Lignocellulose HydrolysisLignocellulosic biomass, primarily comprised of cellulose, hemicellulose, and lignin, is an abundant and renewable resource that holds great promise as a source of biofuels and renewable biobased chemicals and biomaterials. Lignocellulosic biomass can be processed in a number of ways, one is through the hydrolysis of the structural polysaccharides (cellulose and hemicellulose) into their constituent sugars, a reaction commonly facilitated by acid or enzymes, followed by the fermentation of these sugars by yeast or other microorganisms.
Enzymatic HydrolysisIn enzymatic hydrolysis cellulases and hemicellulases play a critical role, working synergistically to cleave the glycosidic linkages in cellulose and hemicellulose, respectively. However, depending on the type of pretreatment process involved, hydrolysis of hemicellulose may not be necessary, since it may have already taken place in the pretreatment leading to the hemicellulose sugars being in the liquid output of the pretreatment with the solid residue mostly containing cellulose (plus lignin, again dependent on the type of pretreatment).
SSCF ProcessSimultaneous Saccharification and Co-Fermentation (SSCF) is a modification of the Simultaneous Saccharification and Fermentation (SSF) method. SSF involves the concurrent breakdown (hydrolysis) of cellulose (and hemicellulose, if present) into monomeric sugars (saccharification), and the conversion of these sugars into products via fermentation. However, SSF often involves the use of a prior pretreatment step where the hemicellulose is removed. Hence, the primary focus of the fermentation in SSF is on the conversion of glucose to the targeted product (e.g. bioethanol).
Fed-Batch SSFAs with SSF, SSCF can also be operated under Fed-batch mode. This is a modification of the traditional SSCF process which can, in certain circumstances, improve process efficiencies.
Advantages of SSCF
Disadvantages of SSCF
1. Understanding Your Requirements
2. Detailed Feedstock Analysis
3. Pretreatment (Lab-Scale)
4. SSCF Optimisation
5. Product Recovery
6. Valorisation of Remaining Biomass
7. Validation at Higher TRLs
8. Technoeconomic Analysis (TEA)
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PhD (Analytical Chemistry)
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