Analysis of Starch
Amylose has an approximate degree of polymerisation (DP - number of monosaccharide units) of 2000 and the polysaccharide forms a helix with six glucose units in each turn. However, amylose is usually a minor component of most starches with amylopectin being the major constituent. Amylopectin again contains glucose units linked through alpha (1-4) bonds; however, there are also alpha (1-6) branches that occur every 24 to 30 glucose units in plants. Amylopectin also has a much higher DP than amylose (e.g. 200,000 residues in potatoes). It cannot coil into a long helix, however, because of the extensive branching, and the fact that the chains that do exist before branching are too short. This means that no compact intermolecular alignment, and hence no significant hydrogen bonding, can take place. This accounts for its extensive solubility, especially when compared with cellulose.
Compared to sucrose, the conversion of starch to ethanol is more complex since the polysaccharide is more resistant to hydrolysis, which means that catalysts are required. However, it is a lot easier to hydrolyse starch than cellulose due to the extensive hydrogen bonding of the latter and the complex lignocellulosic matrix it is contained within.
The main starch-producing feedstocks that are used for biofuel production are wheat (Triticum aestivum) and maize/corn (Zea mays). In a maize to ethanol dry-mill facility the maize is ground to a mash and heated in a cooker where the starch is enzymatically-hydrolysed using amylases and the liberated sugars are then fermented using yeasts. The main products are ethanol, and dried distillers grains which can be used in animal feed.
Maize wet-mills can produce, in addition to ethanol, an array of co-products including oils, proteins, fibre, and nutrients. That is possible because the germ, starch, gluten, and fibre are separated. Furthermore, after hydrolysis, the starch can be enzymatically converted to sweeteners (e.g. a high-fructose content syrup) as an alternative to fermentation to ethanol or other products.
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Analysis Packages for Starch
Total Sugars, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Lignin (Klason), Lignin (Acid Soluble), Carbon, Extractives (Ethanol-Soluble), Extractives (Water-Soluble), Extractives (Exhaustive - Water then Ethanol), Extractives (Water-Insoluble, Ethanol Soluble) , Ash, Ash (Acid Insoluble), Starch, Pectin, Glucuronic Acid, Galacturonic Acid, 4-O-Methyl-D-Glucuronic Acid
Equipment Used for Starch Analysis
Ion Chromatography
A Dionex ICS-3000 system that is equipmed with electrochemical, conductivity, and ultraviolet-visible detectors.
Production of amylase under submerged fermentation Bacillus sp. was investigated using wheat bran, soybean meal and CaCO3 (WSC) medium. Response surface methodology (RSM) was used to evaluate the effect of the main variables, i.e., pH (11.35), temperature (35.16°C) and inoculum size (2.95%) on amylase production by applying a full factorial central composite design (CCD). The mutual interaction between these variables resulted into 4.64 fold increase in amylase activity as compared to the non-optimized environmental factors in the basal medium. | |
Glucoamylase is a well recognized amylolytic enzyme used in food industry, which is generally produced by Aspergillus genus under solid-state fermentation (SSF). This study presents production of glucoamylase by Aspergillus oryzae on the solid surface of rice husk, wheat bran, rice bran, cotton seed powder, corn steep solids, bagasse powder, coconut oil cake, and groundnut oil cake as substrates. Optimization of the SSF media and parameters resulted in a 24% increase in the glucoamylase activity. Optimum glucoamylase production (1986 µmoles of glucose produced per minute per gram of dry fermented substrate) was observed on wheat bran supplemented with 1%, (w/w) starch, 0.25%, (w/w) urea at pH 6, 100%, (v/w) initial moisture and 300C after incubation 120 hrs. Therefore, A. oryzae can be useful in bioprocessing application for saccharification of agro-residues. | |
Additional Material
Lignin (Klason), Lignin (Acid Soluble), Acid Insoluble Residue, Ash (Acid Insoluble),
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid,
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Xylose in Enzyme Hydrolysate, Arabinose in Enzyme Hydrolysate, Mannose in Enzyme Hydrolysate, Galactose in Enzyme Hydrolysate, Rhamnose in Enzyme Hydrolysate, Cellobiose in Enzyme Hydrolysate, Enzymatic Hydrolysis Kinetics, Cellulose Conversion Yield, Xylan Conversion Yield, Combined Sugar Yield, Cellulose Conversion Rate, Xylan Conversion Rate,
As P121 plus comparisons with data from the non-pretreated original sample, including: Increase in Cellulose Accessibility after Pre-Treatment, Percent Increase in Cellulose Conversion Efficiency, Percent Increase in Cellulose Conversion Rate.
As P9 but on the solid residue after enzymatic hydrolysis.
Formic Acid, Acetic Acid, Levulinic Acid, Furfural, Hydroxymethylfurfural,
Includes all hydrolysate sugars and kinetics in P121 and: Cellulose Conversion Yield, Cellulose Conversion Rate
Includes all hydrolysate sugars and kinetics in P121 and: Xylan Conversion Yield, Xylan Conversion Rate
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Maltose in Enzyme Hydrolysate, a-Amylase Hydrolysis Kinetics, Glucoamylase Hydrolysis Kinetics,
Glucose, Xylose, Fructose, Sucrose, Mannose, Arabinose, Galactose, Rhamnose, Xylitol, Sorbitol, Trehalose, Mannitol, Arabinitol, Glycerol, Raffinose,
Fucose, Mannitol, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Total Sugars, Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid,
Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Astaxanthin, Zeaxanthin,
Biomethane Potential (BMP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
Total Solids, Volatile Solids, pH, Chemical Oxygen Demand (COD), Biological Oxygen Demand (BOD), Phosphorus, Potassium, Ammonia, Carbon, Hydrogen, Nitrogen, Sulphur,
Levoglucosan, Cellobiosan, Mannosan, Galactosan, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Fucose, Sucrose, Cellobiose, Total Sugars,
31 constituents including Phenol, Furfural, Syringol, and Vanillin
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (5 Point Using Nitrogen),
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (20 Point Using Carbon Dioxide), Pore Volume, Pore Size Distribution,
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (40 Point Using Nitrogen), Pore Volume, Pore Size Distribution,
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
Moisture, Ash Content (815C), Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Chlorine, Volatile Matter, Fixed Carbon, Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium, Gross Calorific Value, Net Calorific Value, Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
As P393 plus inorganic carbon, organic carbon, TGA (under nitrogen and air), and inherent moisture
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Acenaphthene, Acenaphthylene, Anthracene, Benz[a]anthracene, Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[ghi]perylene, Benzo[a]pyrene, Chrysene, Dibenz[a,h]anthracene, Fluoranthene, Fluorene, Indeno[1,2,3-cd]pyrene, 1-Methylnaphthalene, 2-Methylnaphthalene, Naphthalene, Phenanthrene, Pyrene,
Time to Germination, Mean Shoot Length (Week 1), Mean Shoot Length (Week 2), Mean Shoot Length (Week 3), Mean Shoot Length (Week 4), Shoot Weight (Week 4), Mean Root Length (Week 4), Root Weight (Week 4),
As Deluxe package plus P383, SEM Imaging (P387) and Plant Growth Trials (P388)
Includes everything from P391 (Physical Properties Ultimate), P394 (Thermal Properties Ultimate), and P397 (Soil Amendment Ultimate)
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Volatile Matter, Fixed Carbon, Moisture, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Gross Calorific Value, Net Calorific Value, Chlorine,
Ash Shrinkage Starting Temperature (Oxidising), Ash Deformation Temperature (Oxidising), Ash Hemisphere Temperature (Oxidising), Ash Flow Temperature (Oxidising),
We are looking for top-class applicants to catalyse Celignis's growth.
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ENABLING is a coordinating and supporting action funded by the H2020-RUR-2017-1 call of the European Union's Horizon 2020 programme.
The title of the project is an acronym that stands for 'Enhance New Approaches in BioBased Local Innovation Networks for Growth'. The focus of the project is on supporting the spreading of best practices
and innovation in the provision (production, pre-processing) of biomass for the Bio-Based Industry (BBI).
Celignis will play a key role in the project with regards to stressing the importance of biomass composition in terms of evaluating feedstock and technology suitability.
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