• Analytes Determined at Celignis
    Extractives (Exhaustive - Water then Ethanol)

The Exhaustive Extractives content is considered to be the total mass proportion of the biomass that is lost as a result of a sequential extraction using water and then 95% ethanol.

The Exhaustive Extractives content is equal to the Water-Soluble Extractives content plus the Water-Insoluble but Ethanol-Soluble Extractives content. It is usually not equivalent to the sum of the Water-Soluble Extractives content and the Ethanol-Soluble Extractives content since some constituents may be extracted in both solvents.

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Analysis Packages for Extractives (Exhaustive - Water then Ethanol)

The Celignis Analysis Package(s) that determine this constituent are listed below:

Analytical Procedure for Extractives (Exhaustive - Water then Ethanol)


☑ Step 1: Removal of Water-Soluble and Ethanol-Soluble Extractives

The steps involved in the removal of Water-Soluble and 95%-Ethanol Soluble Extractives from samples are listed below:

1. The moisture content of the sample is determined, in duplicate.

2. Six 11 ml capacity DIONEX ASE (Accelerated Solvent Extraction) cells are filled with recorded weights of the sample.

3. The following DIONEX ASE 200 Method is then used on the first 4 cells:

   Pressure:   1500 psi
   Temperature:   100 celcius
   Preheat Time:   0 mins
   Heat Time:   5 mins
   Static Time:   7 mins
   Flush Volume:   150%
   Purge Time:   150 s
   Static Cycles:   3
   Solvent:   Water


4. The Method listed above is then repeated for cells 3-6 with the only difference being that 95%-Ethanol is used as the solvent.

5. A box, of known weight, is taken and the remaining biomass from the first extraction cell transferred to it. This is repeated for the other cells.

6. After 2 days each box is weighed again and the moisture content of a subsample of the extracted biomass determined (in duplicate).

7. The weight of Water-Soluble Extractives is determined as the mass loss in the biomass sample, due to extraction in the ASE-200 (corrected for moisture), for the first two cells.

8. The weight of 95%-Ethanol-Soluble Extractives is determined as the mass loss in the biomass sample, due to extraction in the ASE-200 (corrected for moisture), for the last two cells.

9. The weight of Exhaustive Extractives is determined as the mass loss in the biomass sample, due to extraction in the ASE-200 (corrected for moisture), for cells 3 and 4.

10. The weight of Water-Insoluble but Ethanol-Soluble Extractives is determined from subtracting the Water-Soluble Extractives content from the Exhaustive Extractives content.

Equipment Used for Extractives (Exhaustive - Water then Ethanol) Analysis



Solvent Extractor

Dionex ASE-200 devices are used to determine the extractives (water-soluble, ethanol-soluble) contents of biomass samples.

Additional Material

Click here to read more about our methods for analysing extractives.

We can determine the Extractives (Exhaustive - Water then Ethanol) content of biomass, click here to learn more about our various biomass analysis methods.



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