• Analytes Determined at Celignis
    Extractives (Water-Soluble)

The Water-Soluble Extractives content is the proportion of the biomass that is lost as a result of extraction with water.

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Analysis Packages for Extractives (Water-Soluble)

The Celignis Analysis Package(s) that determine this constituent are listed below:

Analytical Procedure for Extractives (Water-Soluble)

☑ Step 1: Removal of Water-Soluble Extractives

The steps involved in the removal of water-soluble extractives from samples are listed below:

1. The moisture content of the sample is determined, in duplicate.

2. Two 11 ml capacity DIONEX ASE (Accelerated Solvent Extraction) cells are filled with recorded weights of the sample.

3. The following DIONEX ASE 200 Method is then used on each cell:

   Pressure:   1500 psi
   Temperature:   100 celcius
   Preheat Time:   0 mins
   Heat Time:   5 mins
   Static Time:   7 mins
   Flush Volume:   150%
   Purge Time:   150 s
   Static Cycles:   3
   Solvent:   Water

4. A box, of known weight, is taken and the remaining biomass from the extraction cell transferred to it. This is repeated for the other cell.

5. After 2 days each box is weighed again and the moisture content of a subsample of the extracted biomass determined (in duplicate).

6. The weight of extractives is determined as the mass loss in the biomass sample due to extraction in the ASE-200 (corrected for moisture).

Equipment Used for Extractives (Water-Soluble) Analysis

Solvent Extractor

Dionex ASE-200 devices are used to determine the extractives (water-soluble, ethanol-soluble) contents of biomass samples.

Publications on Extractives (Water-Soluble) By The Celignis Team

Vasudeo Zambare, Archana Zambare, Lew Christopher (2010) Antioxidant and antibacterial activity of extracts from lichen Xanthoparmelia somloensis, native to the Black Hills, South Dakota, USA, International Journal Medical Science and Technology 3(7): 46-51


The present study was carried out to evaluate the antioxidant and antibacterial activity of lichen Xanthoparmelia somloensis, native to the Black Hills in South Dakota, USA. The antioxidant activity of lichen extracts was assessed using the 1,1-diphenyl-2-picrylhydrazyl free radical scavenging assay. The lipid peroxidation reaction of acetone and methanol extracts was inhibited 85% and 81%, respectively A free radical scavenging activity of 77% (acetone extract) and 65% (methanol extract) was determined. The antibacterial activity was assayed against four clinical strains using the agar well diffusion method. Except for Escherichia coli, both extracts were found inhibitory to Streptomyces aureus, Streptococcus pyogenes,and Steptococcus agalactiae with minimum inhibitory concentration values of 0.7-0.9 mg/ml. It was demonstrated that both the antioxidant and antibacterial activities correlated well with the protein to polysaccharide ratio rather than the polyphenol content of the lichen extracts. To the best of our knowledge, this is the first literature report on antibacterial activity from the lichen X.somloensis. The results reported here warrant further investigations to establish the usefulness of X.somloensis in biomedical applications such as treatment of respiratory and urinary tract infections.

Additional Material

Click here to read more about our methods for analysing extractives.

We can determine the Extractives (Water-Soluble) content of biomass, click here to learn more about our various biomass analysis methods.