Notice: Undefined variable: data in /home/n9t4gw6xrzpq/public_html/analyte.php on line 223
Analysis of Sucrose, Sucrose Content of Biomass, Sucrose Content of Liquids - Celignis Biomass Analysis Laboratory
  • Analytes Determined at Celignis
    Sucrose

Sucrose is a disaccharide of glucose and fructose and is the most important disaccharide in plants, occupying much of the mass balance in species such as sugarcane, sugar beets and sweet sorghum. It is often present in the water-soluble carbohydrates in biomass, and we can quantify its concentration in these extractives. We can also quantify the sucrose contents of various liquids.

Click here to place an order for determining Sucrose.

Request a QuoteSucrose Content

Analysis Packages for Sucrose

The Celignis Analysis Package(s) that determine this constituent are listed below:


Notice: Undefined variable: ANALYTICAL_PROC in /home/n9t4gw6xrzpq/public_html/analyte.php on line 963

Equipment Used for Sucrose Analysis



Ion Chromatography

A Dionex ICS-3000 system that is equipmed with electrochemical, conductivity, and ultraviolet-visible detectors.



Solvent Extractor

Dionex ASE-200 devices are used to determine the extractives (water-soluble, ethanol-soluble) contents of biomass samples.

Publications on Sucrose By The Celignis Team

Bedzo, O. K. K., Mandegari, M. and Gorgens, J. F. (2020) Comparison of immobilized and free enzyme systems in industrial production of short-chain fructooligosaccharides from sucrose using a techno-economic approach, Biofuels Bioproducts & Biorefining-Biofpr 14(4): 776-793

Link

Short-chain fructooligosaccharides (scFOS) are nutraceuticals with numerous applications in the food and pharmaceutical industries. The production of scFOS using immobilized biocatalysts offers some functional and technical advantages over free enzyme counterparts. To investigate the economic potential of the immobilized enzyme system relative to the free enzyme system, a techno-economic comparison was conducted on three methods of scFOS production (powder and syrup forms) at a capacity of 2000?t per annum (tpa) by enzymatic synthesis from sucrose: the free enzyme (FE), calcium alginate immobilized enzyme (CAIE), and amberlite IRA 900 immobilized enzyme (AIE) systems. These processes were simulated in Aspen Plus to obtain the mass and energy balances and to estimate the operating and capital costs, followed by economic evaluation and sensitivity analysis. Profitability analysis showed that all three systems are economically viable as their associated minimum selling prices (MSP) were well below the scFOS market price of 5 $ kg-1. However, the FE system was the most profitable with the lowest MSP of 2.61 $ kg-1 because the savings on cost as a result of enzyme immobilization could not offset the additional costs associated with immobilization. Sensitivity analysis demonstrated that total operating cost, fixed capital investment, and internal rate of return (% IRR) have the greatest effects on the MSP. Furthermore, the syrup form of scFOS production leads to 29% less MSP, compared to powder form. In addition, the studied plant capacities of 5000 and 1000?tpa showed 10% and 16% reductions on MSP respectively.

Bedzo, O. K. K., Trollope, K., Gottumukkala, L. D., Coetzee, G., Gorgens, J. F. (2019) Amberlite IRA 900 Versus Calcium Alginate in Immobilization of a Novel , Engineered B-fructofuranosidase for Short-Chain Fructooligosaccharide Synthesis from Sucrose, Biotechnology Progress 35(3): 1-9

Link

The immobilization of B-fructofuranosidase for short-chain fructooligosaccharide (scFOS) synthesis holds the potential for a more efficient use of the biocatalyst. However, the choice of carrier and immobilization technique is a key to achieving that efficiency. In this study, calcium alginate (CA), Amberlite IRA 900 (AI900) and Dowex Marathon MSA (DMM) were tested as supports for immobilizing a novel engineered B-fructofuranosidase from Aspergillus japonicus for scFOS synthesis. Several immobilization parameters were estimated to ascertain the effectiveness of the carriers in immobilizing the enzyme. The performance of the immobilized biocatalysts are compared in terms of the yield of scFOS produced and reusability. The selection of carriers and reagents was motivated by the need to ensure safety of application in the production of food-grade products. The CA and AI900 both recorded impressive immobilization yields of 82 and 62%, respectively, while the DMM recorded 47%. Enzyme immobilizations on CA, AI900 and DMM showed activity recoveries of 23, 27, and 17%, respectively. The CA, AI900 immobilized and the free enzymes recorded their highest scFOS yields of 59, 53, and 61%, respectively. The AI900 immobilized enzyme produced a consistent scFOS yield and composition for 12 batch cycles but for the CA immobilized enzyme, only 6 batch cycles gave a consistent scFOS yield. In its first record of application in scFOS production, the AI900 anion exchange resin exhibited potential as an adequate carrier for industrial application with possible savings on cost of immobilization and reduced technical difficulty.

Additional Material

Click here to learn more about our various methods for analysing oligosaccharides.

We can determine the Sucrose content of biomass, click here to learn more about our various biomass analysis methods.

We can determine the Sucrose content of various liquids, including liquids from pre-treatment and hydrolysis processes, click here to learn more about our various methods for analysing process liquids.



...