• Analytes Determined at Celignis
    Total Oligomeric Sugars

We determine the total oligomeric sugar content of a liquid and also the relative proportions that each monosaccharide contributes to this total amount. This involves a number of stages of analysis.

Firstly, the liquid is analysed using our ion chromatography system for the free monosaccharides and disaccharides in solution, just as in package P21 - Sugars in Solution.

Then we subject the liquid to a mild form of acid hydrolysis involving 4% sulphuric acid and 1 hour in an autoclave at 121 degrees Celcius. These conditions will break apart any oligosaccharides into their constituent monosaccharide units.

The liquid is subsequently filtered and analysed again on our IC system, to determine the amounts of various monosaccharides, after correction for any losses in sugars associated with the hydrolysis process. For each monosaccharide there will now be two values, the concentration prior to hydrolysis and that after hydrolysis.

With the exception of fructose (which degrades to hydroxymethylfurfural during the autoclaving step) all monosaccharides should be present in greater concentrations post-hydrolysis. Hence, the proportion of each sugar that is present in the original liquid in the oligomeric form can be calculated by subtracting the pre-hydrolysis concentration from the post-hydrolysis concentration. As we report the sucrose and cellobiose contents separately we correct the calculated oligomeric glucan content using those data.

In our reports we provide the pre-hydrolysis, post-hydrolysis and oligomeric concentrations of each sugar.


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Analysis Packages for Total Oligomeric Sugars

The Celignis Analysis Package(s) that determine this constituent are listed below:

Equipment Used for Total Oligomeric Sugars Analysis



Ion Chromatography

A Dionex ICS-3000 system that is equipmed with electrochemical, conductivity, and ultraviolet-visible detectors.

Publications on Total Oligomeric Sugars By The Celignis Team

Bedzo, O.K.K, Trollope K, Gottumukkala L.D, Coetzee G, Gorgens J.F. (2019) Amberlite IRA 900 versus calcium alginate in immobilization of a novel, engineered ? fructofuranosidase for short?chain fructooligosaccharide synthesis from sucrose, Biotechnology progress 35(3)

The immobilization of ? fructofuranosidase for short?chain fructooligosaccharide (scFOS) synthesis holds the potential for a more efficient use of the biocatalyst. However, the choice of carrier and immobilization technique is a key to achieving that efficiency. In this study, calcium alginate (CA), Amberlite IRA 900 (AI900) and Dowex Marathon MSA (DMM) were tested as supports for immobilizing a novel engineered ??fructofuranosidase from Aspergillus japonicus for scFOS synthesis. Several immobilization parameters were estimated to ascertain the effectiveness of the carriers in immobilizing the enzyme. The performance of the immobilized biocatalysts are compared in terms of the yield of scFOS produced and reusability. The selection of carriers and reagents was motivated by the need to ensure safety of application in the production of food?grade products. The CA and AI900 both recorded impressive immobilization yields of 82 and 62%, respectively, while the DMM recorded 47%. Enzyme immobilizations on CA, AI900 and DMM showed activity recoveries of 23, 27, and 17%, respectively. The CA, AI900 immobilized and the free enzymes recorded their highest scFOS yields of 59, 53, and 61%, respectively. The AI900 immobilized enzyme produced a consistent scFOS yield and composition for 12 batch cycles but for the CA immobilized enzyme, only 6 batch cycles gave a consistent scFOS yield. In its first record of application in scFOS production, the AI900 anion exchange resin exhibited potential as an adequate carrier for industrial application with possible savings on cost of immobilization and reduced technical difficulty.

Additional Material

Click here to learn more about our various methods for analysing oligosaccharides.

We can determine the Total Oligomeric Sugars content of various liquids, including liquids from pre-treatment and hydrolysis processes, click here to learn more about our various methods for analysing process liquids.

We can determine the Total Oligomeric Sugars content of pyrolysis bio-oils, click here to learn more about our various methods for analysing bio-oil.



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