Downstream ProcessingDownstream-processing in bioprocesses concerns the ways in which output streams (e.g. solid, liquid, slurry etc.) are handled and the means for the recovery and purification of the targeted products. It is a crucial step in bioprocess development that is often overlooked, especially in early stages of research and development, where much of the focus tends to be on optimizing the bioconversion process itself. This is a critical oversight given that downstream processing can account for a large portion (sometimes up to 80%) of the total production costs, particularly in processes dealing with dilute concentrations of the target product or complex mixtures.
Importance in BioprocessesGiving due focus to downstream processing in bioprocess development can not only lead to better product recovery and quality but also to significant cost savings and improved sustainability. This is especially important in processes concerning the valorisation of lignocellulosic feedstocks, where the complexity of the feedstock and the need for high-purity end products can make the downstream process a significant factor in the overall economic viability of the process.
BackgroundTangential Flow Filtration (TFF), also known as cross-flow filtration, has increasingly gained prominence in the field of bioprocess development due to its robustness, scalability, and versatility.
Important Aspects in TFF
Advantages of TFF
Disadvantages of TFF
Millipore ProFlux M30 TFF SystemThe ProFlux M30 Tangential Flow Filtration System can utilise spiral-wound cartridges, hollow fiber cartridges, or cassette membrane modules. The system's control panel displays crucial process data and incorporates safety features that allow manual handling of tangential ultrafiltration and microfiltration applications. The unit allows control over pump speed and backpressures according to the requirrments of the process and membranes.
Millipore Pellicon 2The Millipore Pellicon 2 system is a lab-scale TFF system that uses ultrafiltration and microfiltration cassette membranes, which are characterized by their low hold-up volume, high surface area, and robust performance. These cassette membranes, which are available in a variety of pore sizes and materials, provide effective and scalable solutions for the processing of biological products, including proteins, antibodies, and viruses.
Has a deep understanding of all biological and chemical aspects of bioproceses. Has developed Celignis into a renowned provider of bioprocess development services to a global network of clients.
A dynamic, purpose-driven chemical engineer with expertise in bioprocess development, process design, simulation and techno-economic analysis over several years in the bioeconomy sector.
PhD (Analytical Chemistry)
Dreamer and achiever. Took Celignis from a concept in a research project to being the bioeconomy's premier provider of analytical and bioprocessing expertise.