Bacteria are mainly used to produce organic acids and alcohols by anaerobic fermentation and enzymes by aerobic fermentation processes.
Very well-known natural fermenters are lactic acid bacteria (LAB) for lactic acid production and Bacillus species (such as B. subtilis,
B. amyloliquefaciens, B. licheniformis, B. megaterium etc.) for the production of enzymes, antibiotics, surfactins, and biopolymers.
Here at Celignis our team is highly experienced in numerous types of fermentations using bacteria
and can help you determine and optimise
the potenital yields of an array of different fermentation products. If you already have a technology, e.g. pre-treatment and/or
hydrolysis, that produces a sugars-containing liquid then we can undertake fermentation tests directly on that.
Alternatively, if you are starting with a feedstock and
looking for the best approach to get your targetted fermentation product(s) in high yield then we can help you optimise the whole process,
covering pre-treatment, hydrolysis and the subsequent fermentation.
Lactic acid bacteria are very sensitive and require complex nutrient media compared to other bacillus species that can produce lactic acid.
Hence, industries are constantly looking for fungi and bacillus strains that have low nutrient requirements and can tolerate acidic pH.
At Celignis we have expertise and experience in screening lactic acid bacteria for the selection of substrate- and product-tolerant strains. We can also develop:
fed-batch strategies to achieve high cell mass, and in situ product recovery techniques to separate lactic acid from the fermentation broth. We will
work with you and develop bespoke lactic acid fermentation methods for your feedstock or industrial waste streams.
Propionic acid can be produced from a variety of substrates such as glucose, ethanol, lactose, glycerol, and pectin. So, several industrial streams
will be suitable to produce propionic acid, if the bacteria are adapted to the inhibitors present in the waste streams and fermentation is optimised to
achieve high cell densities and high product concentration.
We can perform anaerobic fermentations and develop fermentation strategies to achieve high cell mass and in-situ product recovery techniques.
We can screen your feedstock for propionic acid production, adapt the strain to any inhibitors present in the feed, and develop bespoke fermentation
and product recovery processes.
Butyric acid is biologically produced by Clostridium species and like other acids (acetic acid, lactic acid, propionic acid),
it is toxic to the bacteria after a certain concentration. Hence, the product titres are generally low which makes downstream expensive.
In order to reduce these costs, in situ removal of butyric acid can be developed. In situ removal strategies are not yet industrially applied for butyric acid, but
it is a key area where progress has to be made to make the process economically sustainable.
At Celignis, we have strong expertise in Clostridial fermentation. We can isolate and or adapt the strains that are suitable for your feedstock and can
develop fermentation strategies to reduce substrate and product inhibition. We will innovate with you for you.
Butanol fermentation is also one of the difficult fermentation pathways due to substrate and product inhibition. However, this can be
avoided by fed-batch fermentation and in-situ stripping of butanol. Also, reducing the feedstock and enzyme costs will make the process more
economically viable. Through using industrial waste streams (negative costs), enzymatic cocktails tailored for the feedstock (allowing low-enzyme dosages), and
with high sugar yields, the right choice of microbial strain, and an effective in-situ removal technology, it is possible to develop an economically-viable butanol process.
At Celignis, we have considerable expertise in Clostridial fermentation and especially butanol fermentation.
Our Chief Innovation Officer Dr Lalitha Gottumukkala has extensively worked in this area and has isolated novel strains and developed novel
methods for non-acetogenic butanol fermentation as part of her PhD.
Natural microbes that produce 1,3-Propanediol are Klebsiella, Clostridia, Citrobacter, Enterobacter
and Lactobacilli. They all use glycerol as a carbon source and produce 1,3-PDO through 3-hydroxypropionaldehyde route
using glycerol dehydratase enzymes and 1,3-propanediol oxidoreductase enzymes.
At Celignis, we have expertise and experience in performing anaerobic fermentations
and developing fermentation strategies to achieve high cell mass and in situ product recovery techniques. We can screen your feedstock for 1,3-Propanediol production,
adapt the strain to any inhibitors present in the feed, and develop bespoke fermentation and product recovery process.
PHA is one of the most complicated fermentation processes, but the possibility to use mixed microbial cultures and the avoidance of sterilisation costs
makes it an interesting process to produce bioplastics. Also, PHA blends are becoming more and more popular to increase the tensile strength and
flexibility of the polymer, possible by using mixed culture substrates.
At Celignis, we have experience in enrichment of desired microorganisms, fed-batch and continuous fermentations
with cell-recycling. We can design and develop the most suitable process for your feedstock by using mixed or mono-culture fermentations.
We can also develop cost-efficient downstream processing steps for efficient PHA extraction by using non-toxic and environmentally friendly techniques.
Yeast fermentation is one of the oldest fermentations and is used in everyday life to produce a variety of commodity products including bread, beer, wine,
cheese, and soy sauce. A few decades ago, yeast gained popularity as an industrial strain for biorefinery and biofuel applications.
Algal cultivation is complicated and requires optimisation to achieve high biomass yields. Algal biomass production depends on nutrient uptake and
other environmental conditions such as temperature, pH, salt concentration etc. It is important to select the strain based on the type of
production (open ponds, photobioreactors), feedstock and application. We have particular expertise in the evaluation and optimisation of algae thorugh
our Chief Innovation Officer, Lalitha, who is currently undertaking a Marie-Curie funded project at Celignis on this topic.
We are available to answer any questions you may have on how to get high value chemicals and biofuels from biomass through fermentation processes.
Just get in touch with us by sending us an email email@example.com, giving us a call at (+353) 61 371 725, or through
our contact form.
We are looking for top-class applicants to develop bioprocessing IP at Celignis
We are pleased to announce that we have been selected to be awarded funding, through the Horizon 2020 Innosup Innovation Associate programme, to recruit a top-class person to lead the development of our bioprocess concept into a patentable process and prototype product with clear commercial potential.
The SAPHIRE (Self-Assembling Plant-based Hydrogels Induced by Redox Enzymes) project focuses on the production of environmentally-friendly, 100% plant-based, superior-quality hydrogels for food,
cosmetic and pharmaceutical applications.
The position has a salary of €69.5k for one year, plus €20k of training and €3.5k in relocation funds.
Please click here for further information on the position and how to apply.
Celignis Collaborating with Ibiocat on Biorefinery Solutions
Illinois-based Ibiocat, was founded by Charles Abbas, a leading light for over 40 years in biorefining.
Illinois-based Ibiocat, founded by Charles A. Abbas, and Ireland-based analytical provider Celignis, founded by Dan Hayes, have come together to develop bespoke bioeconomy solutions for clients that are looking to add value to their process residues generated from 1G and 2G ethanol plants.
Click here to read more about this exciting collaboration and here to download a promotional flyer.
We're Hosting a Review Meeting of H2020 Project ENABLING
The 2 day event will see all 16 partners of the ENABLING project discuss the progress to date.
This two-day event will see all 16 project partners discuss the progress made in the first 18 months of our Horizon 2020 project ENABLING and make plans for the activities to be undertaken in the second half of the project.
The focus of the project is on supporting the spreading of best practices and innovation in the provision (production, pre-processing) of biomass for the Bio-Based Industry (BBI).
Details the latests activities and findings of the ENABLING project
We are happy to announce that the 4th newsletter of the ENABLING project has been released.
ENABLING is a coordinating and supporting action funded by the H2020-RUR-2017-1 call of the European Union's Horizon 2020 programme.
The title of the project is an acronym that stands for 'Enhance New Approaches in BioBased Local Innovation Networks for Growth'. The focus of the project is on supporting the spreading of best practices
and innovation in the provision (production, pre-processing) of biomass for the Bio-Based Industry (BBI).
Celignis will play a key role in the project with regards to stressing the importance of biomass composition in terms of evaluating feedstock and technology suitability.
Over the course of the project we will also be contacting a number of stakeholders, both in Ireland and overseas, and will be involved in the organisation of a number of networking events.
Thanks for contacting us. One of our representatives will be in contact with you shortly regarding your inquiry. If you ever have any questions that require immediate assistance, please call us at +353 61 371 725.
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