Services for the Analysis of Sugar Degradation Products
Background
Particularly when elevated temperatures and/or chemicals are employed in either pre-treatment or hydrolysis it can be possible for the production of the monosaccharide to not be the end-point of the process. Some sugars may be further degraded to a variety of potential products. This is important since these products can often be inhibitory to fermentation or can otherwise complicate subsequent downstream processing methods.
Celignis personnel have significant experience in the analysis of sugar degradation products. The company was formed based on the outputs of a European research project called DIBANET that targeted the production of the degradation products levulinic acid, furfural, hydroxymethylfurfural, and formic acid from lignocellulosic biomass. We have developed analytical methods to determine the concentrations of the main degradation products of interest. These can be categorised according to two main classes, organic acids and furans.
Levulinic Acid
One derivative of levulinic acid is gamma-Valerloactone, which we can also quantify using our ion chromatography system as well as by using our our GC-MS system.
Formic Acid
Formic acid is used extensively as a decalcifier, as an acidulating agent in textile dying and finishing, and in leather tanning. It is also used in the preparation of organic esters and in the manufacture of drugs, dyes, insecticides, and refrigerants. In industrial production, formic acid is usually produced as a by-product of acetic acid production by liquid phase oxidation of hydrocarbons.
Acetic Acid
Analysis of Furans
Furfural
Hydroxymethylfurfural
Analysis of Organic Acids and Furans at Celignis
Analysis package P22 - Organic Acids and Furans can also be used to determine the amounts of organic acids and furans in other substances, for example the hydroxymethylfurfural content of honey. The chemicals determined by analysis package P22 are detailed below:
Request a QuoteSugar Degradation Products
Lignin (Klason), Lignin (Acid Soluble), Acid Insoluble Residue, Ash (Acid Insoluble),
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid,
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Xylose in Enzyme Hydrolysate, Arabinose in Enzyme Hydrolysate, Mannose in Enzyme Hydrolysate, Galactose in Enzyme Hydrolysate, Rhamnose in Enzyme Hydrolysate, Cellobiose in Enzyme Hydrolysate, Enzymatic Hydrolysis Kinetics, Cellulose Conversion Yield, Xylan Conversion Yield, Combined Sugar Yield, Cellulose Conversion Rate, Xylan Conversion Rate,
As P121 plus comparisons with data from the non-pretreated original sample, including: Increase in Cellulose Accessibility after Pre-Treatment, Percent Increase in Cellulose Conversion Efficiency, Percent Increase in Cellulose Conversion Rate.
As P9 but on the solid residue after enzymatic hydrolysis.
Formic Acid, Acetic Acid, Levulinic Acid, Furfural, Hydroxymethylfurfural,
Includes all hydrolysate sugars and kinetics in P121 and: Cellulose Conversion Yield, Cellulose Conversion Rate
Includes all hydrolysate sugars and kinetics in P121 and: Xylan Conversion Yield, Xylan Conversion Rate
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Maltose in Enzyme Hydrolysate, a-Amylase Hydrolysis Kinetics, Glucoamylase Hydrolysis Kinetics,
Glucose, Xylose, Fructose, Sucrose, Mannose, Arabinose, Galactose, Rhamnose, Xylitol, Sorbitol, Trehalose, Mannitol, Arabinitol, Glycerol, Raffinose,
Fucose, Mannitol, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Total Sugars, Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid,
Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Astaxanthin, Zeaxanthin,
Biomethane Potential (BMP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
Total Solids, Volatile Solids, pH, Chemical Oxygen Demand (COD), Biological Oxygen Demand (BOD), Phosphorus, Potassium, Ammonia, Carbon, Hydrogen, Nitrogen, Sulphur,
Levoglucosan, Cellobiosan, Mannosan, Galactosan, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Fucose, Sucrose, Cellobiose, Total Sugars,
31 constituents including Phenol, Furfural, Syringol, and Vanillin
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Volatile Matter, Fixed Carbon, Moisture, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Gross Calorific Value, Net Calorific Value, Chlorine,
Ash Shrinkage Starting Temperature (Oxidising), Ash Deformation Temperature (Oxidising), Ash Hemisphere Temperature (Oxidising), Ash Flow Temperature (Oxidising),
We are looking for top-class applicants to catalyse Celignis's growth.
We are looking for someone to join the Celignis team and spearhead our growth and expansion into new markets and territories.
The position has a salary of €69.5k for one year, plus €20k of training and €3.5k in relocation funds.
Please click here for further information on the position and how to apply.
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The SAPHIRE (Self-Assembling Plant-based Hydrogels Induced by Redox Enzymes) project focuses on the production of environmentally-friendly, 100% plant-based, superior-quality hydrogels for food,
cosmetic and pharmaceutical applications.
The position has a salary of €69.5k for one year, plus €20k of training and €3.5k in relocation funds.
Please click here for further information on the position and how to apply.
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Illinois-based Ibiocat, was founded by Charles Abbas, a leading light for over 40 years in biorefining.
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Click here to read more about this exciting collaboration and here to download a promotional flyer.
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Click here for more information on ENABLING.
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ENABLING is a coordinating and supporting action funded by the H2020-RUR-2017-1 call of the European Union's Horizon 2020 programme.
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and innovation in the provision (production, pre-processing) of biomass for the Bio-Based Industry (BBI).
Celignis will play a key role in the project with regards to stressing the importance of biomass composition in terms of evaluating feedstock and technology suitability.
Over the course of the project we will also be contacting a number of stakeholders, both in Ireland and overseas, and will be involved in the organisation of a number of networking events.
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