Analysis of Betalains
Background
Betalains are divided into two categories:
Betacyanins: These pigments range in colour from violet to red. Betanin is the most common betacyanin and is responsible for the deep red colour of beets.
Betaxanthins: These pigments are yellow to orange. Indicaxanthin is a common betaxanthin found in plants like beets and Swiss chard.
- Pigmentation - Betalains provide bright red to violet and yellow colours, playing a vital role in attracting pollinators.
- Protection - Betalains help protect the plant from UV radiation damage and are involved in plant response to stress.
In addition to their roles in plants, betalains are valued for their potential health benefits. They have antioxidant properties, meaning they can protect cells from damage by harmful free radicals. They may also have anti-inflammatory effects and may contribute to detoxification processes in the body. As such, they are often used as natural food colourants and in nutritional supplements.
Betalains in Different Types of Biomass
- Amaranthin - This is a betacyanin that is found in the vibrant flowers of certain Amaranthus species.
- Betanin - This betacyanin is found in the petals of some species of ice plant (Delosperma).
- Vulgaxanthin - This is a betaxanthin found in the flowers of Mirabilis jalapa.
- Miraxanthiun - Another betaxanthin that can be found in flowers.
Get more info...Flowers
- Beets - One of the richest sources of betalains, especially betacyanins, which give them their characteristic deep red color. Beets also contain betaxanthins.
- Swiss Chard - Both the red and yellow varieties of Swiss chard contain betalains. Red Swiss chard is rich in betacyanins, while yellow Swiss chard contains betaxanthins.
- Prickly Pear - lso known as cactus fruit, prickly pears contain both betacyanins and betaxanthins. The exact amount and type depend on the variety, with red-purple varieties containing more betacyanins and yellow-orange varieties containing more betaxanthins.
- Pitahaya (Dragon Fruit) - This tropical fruit, especially the red-fleshed variety, contains betacyanins.
- Golden Beets - These contain betaxanthins, which provide their yellow color.
- Rhubarb - The red stalks of rhubarb contain betacyanins.
Get more info...Fruit & Veg
Extraction of Betalains from Biomass
At Celignis we have extensive experience in the design and optimisation of extraction protocols for a wide variety of chemical constituents from many different biomass types. If needed we can also work on processes to separate and purify the target compounds.
Click here to read more about Celignis's Bioprocess Development Services for the extraction of chemicals from biomass.
Get more info...Biomass Extractions
Analysis of Betalains at Celignis
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Request a QuoteBetalains Analysis
Lignin (Klason), Lignin (Acid Soluble), Acid Insoluble Residue, Ash (Acid Insoluble),
As P10 plus protein-corrected lignin, water-soluble sugars, uronic acids, acetyl content and starch.
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid, Iduronic Acid,
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Xylose in Enzyme Hydrolysate, Arabinose in Enzyme Hydrolysate, Mannose in Enzyme Hydrolysate, Galactose in Enzyme Hydrolysate, Rhamnose in Enzyme Hydrolysate, Cellobiose in Enzyme Hydrolysate, Enzymatic Hydrolysis Kinetics, Cellulose Conversion Yield, Xylan Conversion Yield, Combined Sugar Yield, Cellulose Conversion Rate, Xylan Conversion Rate,
As P121 plus comparisons with data from the non-pretreated original sample, including: Increase in Cellulose Accessibility after Pre-Treatment, Percent Increase in Cellulose Conversion Efficiency, Percent Increase in Cellulose Conversion Rate.
As P9 but on the solid residue after enzymatic hydrolysis.
Formic Acid, Acetic Acid, Levulinic Acid, Furfural, Hydroxymethylfurfural,
Includes all hydrolysate sugars and kinetics in P121 and: Cellulose Conversion Yield, Cellulose Conversion Rate
Includes all hydrolysate sugars and kinetics in P121 and: Xylan Conversion Yield, Xylan Conversion Rate
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Maltose in Enzyme Hydrolysate, a-Amylase Hydrolysis Kinetics, Glucoamylase Hydrolysis Kinetics,
Glucose, Xylose, Fructose, Sucrose, Mannose, Arabinose, Galactose, Rhamnose, Xylitol, Sorbitol, Trehalose, Mannitol, Arabinitol, Glycerol, Raffinose,
Levulinic Acid, Formic Acid, Hydroxymethylfurfural, Furfural, Acetic Acid, gamma-Valerolactone,
Xylobiose, Xylotriose, Arabinobiose, Arabinotriose,
Maltose, Maltotriose, Maltotetraose, Maltopentaose, Maltohexaose, Maltoheptaose, Maltooctaose,
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid, Iduronic Acid,
Gibberellic Acid, Indole-3-acetic acid, Indole-2-acetic acid, Indole-3-propionic acid, Indole-3-butyric acid, 6-Benzylaminopurine, Kinetin riboside, Abscisic acid, Salicylic acid,
beta-Carotene, Ergocalciferol (Vitamin D2), Alpha-tocopherol (vitamin E), Phylloquinone (Vitamin K1),
Thiamine (Vitamin B1), Riboflavin (Vitamin B2), Niacin (Vitamin B3), Niacinamide (vitamin B3), Pantothenic Acid (Vitamin B5), Pyridoxine (Vitamin B6), Folate (Vitamin B9), Cobalamin (Vitamin B12), Ascorbic Acid (Vitamin C),
Fucose, Mannitol, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Total Sugars, Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, Iduronic Acid,
Alanine, Arginine, Aspartic Acid, Asparagine, Cystine, Glutamic Acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, Valine,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Arachidic Acid, Behenic Acid, Decanoic Acid, Erucic Acid, Lauric Acid, Linoleic Acid, Linolenic Acid, Myristic Acid, Caprylic Acid, Oleic Acid, Palmitic Acid, Palmitoleic Acid, Stearic Acid, Lignoceric Acid,
Fucoxanthin, Astaxanthin, Chlorophyll-c, Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Antheraxanthin, Violaxanthin,
Gibberellic Acid, Indole-3-acetic acid, Indole-2-acetic acid, Indole-3-propionic acid, Indole-3-butyric acid, 6-Benzylaminopurine, Kinetin riboside, Abscisic acid, Salicylic acid,
beta-Carotene, Ergocalciferol (Vitamin D2), Alpha-tocopherol (vitamin E), Phylloquinone (Vitamin K1),
Thiamine (Vitamin B1), Riboflavin (Vitamin B2), Niacin (Vitamin B3), Niacinamide (vitamin B3), Pantothenic Acid (Vitamin B5), Pyridoxine (Vitamin B6), Folate (Vitamin B9), Cobalamin (Vitamin B12), Ascorbic Acid (Vitamin C),
Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Astaxanthin, Zeaxanthin, Antheraxanthin, Violaxanthin,
Biomethane Potential (BMP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
Total Solids, Volatile Solids, pH, Chemical Oxygen Demand (COD),
Residual Biogas Potential (RBP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
Acetic Acid, Lactic Acid, Propionic Acid, Butyric Acid, Isobutyric Acid, Valeric Acid, Isovaleric Acid,
Levoglucosan, Cellobiosan, Mannosan, Galactosan, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Fucose, Sucrose, Cellobiose, Total Sugars,
31 constituents including Phenol, Furfural, Syringol, and Vanillin
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (5 Point Using Nitrogen),
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (20 Point Using Nitrogen), Pore Volume (Using Nitrogen), Pore Size Distribution (Using Nitrogen), Average Pore Width (Using Nitrogen),
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (40 Point Using Nitrogen), Pore Volume (Using Nitrogen), Pore Size Distribution (Using Nitrogen), Average Pore Width (Using Nitrogen),
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
Moisture, Ash Content (815C), Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Chlorine, Volatile Matter, Fixed Carbon, Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium, Gross Calorific Value, Net Calorific Value, Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
As P393 plus inorganic carbon, organic carbon, TGA (under nitrogen and air), and inherent moisture
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Acenaphthene, Acenaphthylene, Anthracene, Benz[a]anthracene, Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[ghi]perylene, Benzo[a]pyrene, Chrysene, Dibenz[a,h]anthracene, Fluoranthene, Fluorene, Indeno[1,2,3-cd]pyrene, 1-Methylnaphthalene, 2-Methylnaphthalene, Naphthalene, Phenanthrene, Pyrene,
Time to Germination, Mean Shoot Length (Week 1), Mean Shoot Length (Week 2), Mean Shoot Length (Week 3), Mean Shoot Length (Week 4), Shoot Weight (Week 4), Mean Root Length (Week 4), Root Weight (Week 4),
As Deluxe package plus P383, SEM Imaging (P387) and Plant Growth Trials (P388)
Includes everything from P391 (Physical Properties Ultimate), P394 (Thermal Properties Ultimate), and P397 (Soil Amendment Ultimate)
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Volatile Matter, Fixed Carbon, Moisture, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Gross Calorific Value, Net Calorific Value, Chlorine,
Ash Shrinkage Starting Temperature (Oxidising), Ash Deformation Temperature (Oxidising), Ash Hemisphere Temperature (Oxidising), Ash Flow Temperature (Oxidising),
We have secured funding for involvement in 4 collaborative research projects
We are delighted to announce that Celignis has been successful in 4 project proposals submitted for funding to the Circular Bio-based Europe Joint Undertaking (CBE JU) programme.
These projects will provide funding of 1.6m EUR to Celignis over the next few years and build upon the 3 projects (worth 1.5m EUR) we secured last year and the 4 previous CBE/BBI projects that Celignis participated in.
Details on the projects are provided below:
WoodVALOR - This RIA project concerns the valorisation of contaminated/post-consumer wood waste (WW) via: (i) thermal conversion to biochar; and (ii) fractionation followed by conversions to paints & coatings ingredients. Celignis is involved in the chemo-enzymatic fractionation of decontaminated wood (DW) to sequentially extract/purify lignin and hemicellulose, and in developing hemicellulose-based emulsifiers/stabilizers and binder monomers for industrial formulations. Additionally, Celignis is involved in metals/mineral recovery from decontamination wastewater using
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The article, available in "Biomass Conversion and Biorefinery" is entitled "Process development for efficient pectin extraction from tobacco residues and its characterisation"
We are please to announce the publication of a peer-reviewed scientific article based on some of the research outputs of a Bioprocess Development Service (BDS) project undertaken by Celignis.
The article, entitled "Process development for efficient pectin extraction from tobacco residues and its characterisation" details the results of experiments targeting the optimised extraction of pectin from the laminae of a number of different varieties of tobacco plants. These tobacco-derived pectins were found to have a medium molecular weight and low methoxy content and our findings indicated that this feedstock could be suitable for the production of pectin with dietary applications.
Click here to view the publication.
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Meeting takes place at the coordinator's (ITA) headquarters in Zaragoza, SPAIN
Celignis personnel are today attending the kick-off meeting of the CBE-JU project MANUREFINERY at the facilities of the project's coordinator (ITA) in Zaragoza, Spain.
MANUREFINERY concerns the development of a small, decentralised, modular biorefinery concept for farms that converts manure and ammonia emissions into seven marketable bio-ingredients (animal-feed proteins, caproic acid, and fertiliser salts/ashes). The solution integrates fixed/mobile units across three valorisation lines (gas, liquid, solid) and a digital twin for optimisation and scale-up, targeting TRL6-7 validation on four EU demo farms.
Celignis has a number of key roles in the project, including:
- Comprehensive analysis of the feedstocks and products of the process.
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Meeting takes place at the coordinator's (AIMPLAS) headquarters in Valencia, Spain
PROMOFER, is an Innovation Action project funded by the CBE-JU, under topic HORIZON-JU-CBE-2023-IA-03 (Improve Fermentation Processes (Including Downstream Purification) To Final Bio-Based Products).
This project started in June 2024 with Celignis, an SME partner and full industry BIC member, playing a pivotal role in the project. Our core activities include undertaking the pre-treatment and hydrolysis of lignocellulosic biomass at scaled-up (TRL7, 1 m3) volumes. The resulting sugars are then provided to other partners for downstream fermentations.
Today Celignis's CIO Lalitha is attending the kick-off meeting of the project, at coordinator AIMPLAS's headquarters in Valencia, Spain.
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We're attending the kick-off meeting for BIONEER in Trondheim, Norway (SINTEF)
Lalitha is attending the kick-off meeting of our CBE-JU project BIONEER, located at the coordinator's (SINTEF) premises in Trondheim, Norway.
BIONEER has the title "Scaled-up Production of Next-Generation Carbohydrate-Derived Building Blocks to Enhance the Competitiveness of a Sustainable European Chemicals Industry". It is a 4-year Innovation Action project with 7.5m EUR of funding provided by the CBE-JU.
Celignis plays a key role in BIONEER, being responsible for the scaled-up (TRL7) production of platform chemicals.
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