Analysis Package P4:
Ethanol Extractives
Ethanol Extractives
Constituents Determined
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Sample Weight Requirements
Under nornal conditions there is a minimum requirement of 15g of sample for this analysis package with a recommended weight of 50+g of sample.However, it may be possible for us to undertake the anaysis with lower quantities than the minimum specified above. Please email us at info@celignis.com to find out.
Analytical Procedure for Ethanol Extractives
Step 1: Removal of Ethanol-Soluble Extractives
The steps involved in the removal of 95% ethanol-soluble extractives from samples are listed below:
1. The moisture content of the sample is determined, in duplicate.
2. Two 11 ml capacity DIONEX ASE (Accelerated Solvent Extraction) cells are filled with recorded weights of the sample.
3. The following DIONEX ASE 200 Method is then used on each cell:
4. A box, of known weight, is taken and the remaining biomass from the extraction cell transferred to it. This is repeated for the other cell.
5. After 2 days each box is weighed again and the moisture content of a subsample of the extracted biomass determined (in duplicate).
6. The weight of extractives is determined as the mass loss in the biomass sample due to extraction in the ASE-200 (corrected for moisture).
1. The moisture content of the sample is determined, in duplicate.
2. Two 11 ml capacity DIONEX ASE (Accelerated Solvent Extraction) cells are filled with recorded weights of the sample.
3. The following DIONEX ASE 200 Method is then used on each cell:
| Pressure: | 1500 psi |
| Temperature: | 100 celcius |
| Preheat Time: | 0 mins |
| Heat Time: | 5 mins |
| Static Time: | 7 mins |
| Flush Volume: | 150% |
| Purge Time: | 150 s |
| Static Cycles: | 3 |
| Solvent: | 95% Ethanol |
4. A box, of known weight, is taken and the remaining biomass from the extraction cell transferred to it. This is repeated for the other cell.
5. After 2 days each box is weighed again and the moisture content of a subsample of the extracted biomass determined (in duplicate).
6. The weight of extractives is determined as the mass loss in the biomass sample due to extraction in the ASE-200 (corrected for moisture).
Equipment Used for Ethanol Extractives
| Solvent Extractor Dionex ASE-200 devices are used to determine the extractives (water-soluble, ethanol-soluble) contents of biomass samples. |
