Extractives (Ethanol-Soluble)
The Ethanol-Soluble Extractives content is the proportion of the biomass that is lost as a result of extraction with 95% ethanol.
Click here for more information on the extractives fraction of biomass.
Click here to place an order for determining Extractives (Ethanol-Soluble).
Analysis Packages for Extractives (Ethanol-Soluble)
The Celignis Analysis Package(s) that determine this constituent are listed below:
P4 Ethanol Extractives |
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| Extractives (Ethanol-Soluble) |
P6 Full Extractives |
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| Extractives (Ethanol-Soluble), Extractives (Water-Soluble), Extractives (Exhaustive - Water then Ethanol), Extractives (Water-Insoluble, Ethanol Soluble) |
Analytical Procedure for Extractives (Ethanol-Soluble)
Step 1: Removal of Ethanol-Soluble Extractives
The steps involved in the removal of 95% ethanol-soluble extractives from samples are listed below:
1. The moisture content of the sample is determined, in duplicate.
2. Two 11 ml capacity DIONEX ASE (Accelerated Solvent Extraction) cells are filled with recorded weights of the sample.
3. The following DIONEX ASE 200 Method is then used on each cell:
4. A box, of known weight, is taken and the remaining biomass from the extraction cell transferred to it. This is repeated for the other cell.
5. After 2 days each box is weighed again and the moisture content of a subsample of the extracted biomass determined (in duplicate).
6. The weight of extractives is determined as the mass loss in the biomass sample due to extraction in the ASE-200 (corrected for moisture).
1. The moisture content of the sample is determined, in duplicate.
2. Two 11 ml capacity DIONEX ASE (Accelerated Solvent Extraction) cells are filled with recorded weights of the sample.
3. The following DIONEX ASE 200 Method is then used on each cell:
| Pressure: | 1500 psi |
| Temperature: | 100 celcius |
| Preheat Time: | 0 mins |
| Heat Time: | 5 mins |
| Static Time: | 7 mins |
| Flush Volume: | 150% |
| Purge Time: | 150 s |
| Static Cycles: | 3 |
| Solvent: | 95% Ethanol |
4. A box, of known weight, is taken and the remaining biomass from the extraction cell transferred to it. This is repeated for the other cell.
5. After 2 days each box is weighed again and the moisture content of a subsample of the extracted biomass determined (in duplicate).
6. The weight of extractives is determined as the mass loss in the biomass sample due to extraction in the ASE-200 (corrected for moisture).
Equipment Used for Extractives (Ethanol-Soluble) Analysis
| NIR Spectrophotometers We have several FOSS XDS NIR devices. These have solid content modules, that can allow for samples of a heterogenous particle to be analysed, and liquid modules that allow liquids to be analysed via transmittance spectroscopy. |
| Solvent Extractor Dionex ASE-200 devices are used to determine the extractives (water-soluble, ethanol-soluble) contents of biomass samples. |
NIR Model
Ethanol-Soluble Extractives Global 1
This model has been built from a global dataset comprising a wide variety of biomass samples. Click here for a list of the types of samples that were used to build this model.
The samples were air-dried and then ground-down to a particle size of less than 850 microns prior to NIR analysis.
Model Statistics
| Min. Value (%) | 0.00 |
|---|---|
| Max. Value (%) | 33.24 |
| Calibration Samples | 472 |
| Validation Samples | 163 |
| R2 (Validation) | 0.882 |
| RMSEP (%) | 1.725 |
| Bias (%) | -0.08 |
| SEP (%) | 1.728 |
| RPD | 2.827 |
| RER | 18.799 |
Regression Plot
(Independent Validation Set)
Additional Material
Click here to read more about our methods for analysing extractives.We can determine the Extractives (Ethanol-Soluble) content of biomass, click here to learn more about our various biomass analysis methods.
